How Vitamins C and E Work Synergistically for Skin Aging: Antioxidants, Collagen & Wrinkle Prevention

Vitamins C and E are powerful antioxidants known for their essential roles in skin health and rejuvenation. As antioxidants, they work to neutralize free radicals—unstable molecules that can cause oxidative stress and accelerate skin aging.

Individually, each of these vitamins offers significant benefits, but together, they create a stronger, synergistic effect that enhances their protective impact on the skin. 

This powerful combination provides a robust defense against environmental stressors like UV radiation and pollution, which are major contributors to premature wrinkles, age spots, and loss of elasticity. Vitamin C is also a potent stimulator of collagen production, helping to keep the skin firm and smooth, while Vitamin E promotes moisture retention and aids in repairing UV-induced damage.

Beyond skin health, this dynamic duo supports other vital processes in the body, including cardiovascular function. In this article, we'll explore the powerful synergy between Vitamins C and E in more detail.

Understanding Antioxidants: Vitamin C and E

Antioxidants are powerful compounds that protect cells from oxidative damage caused by free radicals—unstable oxygen or nitrogen molecules. During cellular respiration, oxygen and glucose are converted into energy, producing reactive oxygen species (ROS) like superoxide (O₂⁻), hydrogen peroxide (H₂O₂), and hydroxyl radicals (HO⁻) as byproducts. 

There are several ways that cells control levels of ROS in the cytosol (the jelly-like fluid inside a cell). One method is by using antioxidant enzymes, such as superoxide dismutase and catalase, to convert these harmful radicals into water and oxygen. In addition to these specialized enzymes, cells also rely on dietary antioxidants, particularly vitamins C and E, for protection (1).

Vitamin E, specifically in its α-tocopherol form, protects cell membranes from lipid peroxidation by neutralizing lipid radicals in the lipid bilayer, effectively breaking the chain reaction that propagates lipid damage. Vitamin E donates an electron to lipid peroxyl radicals formed due to the interaction of free radicals and lipids in the membrane. This process neutralizes the lipid peroxyl radical, converting it into a stable lipid hydroperoxide, while Vitamin E itself becomes a relatively stable tocopheroxyl radical.

These tocopheroxyl radicals can be recycled back to their active form with the help of other antioxidants, particularly Vitamin C. Vitamin C donates an electron to the tocopheroxyl radical, regenerating active Vitamin E and forming dehydroascorbic acid.

Vitamin E radical + Vitamin C → active Vitamin E + Dehydroascorbic Acid

Thereby the both Vitamin E and Vitamin C cycle further ensures a continuous supply of active antioxidants within the cell membrane, maintaining cellular integrity and protecting against oxidative stress-induced damage (2).

Besides the cell's respiration, production of ROSs can be stimulated by environmental stressors like ultraviolet (UV) rays and pollution—damaging cellular structures. When it comes to skin health, antioxidants are essential for neutralizing these free radicals, which helps prevent premature aging, reduce skin damage, and maintain a youthful, radiant appearance. 

Vitamin E has unique moisturizing and repairing properties. It strengthens the skin barrier, allowing the skin to retain moisture more effectively. When applied topically, Vitamin E helps repair damage caused by environmental stressors, particularly UV radiation, which can cause premature aging and hyperpigmentation. Known for its soothing effects, Vitamin E is particularly beneficial for dry, sensitive or irritated skin as it helps to calm the skin and restore balance (3).

Vitamin C is well known for its ability to brighten the complexion, maintain an even skin tone and stimulate collagen production. Collagen is essential for maintaining skin structure and elasticity, so increasing its production helps to maintain skin firmness and reduce the appearance of fine lines and wrinkles (4). Vitamin C also provides protection against photo-aging, or sun damage, by helping to protect the skin from UV radiation (5).

Combating Skin Aging with Vitamin C and E

The battle against skin aging is significantly bolstered by the combined power of Vitamin C and E. Together, these vitamins combat oxidative stress and inflammation—two key contributors to premature skin aging. 

Preventing Wrinkles and Age Spots

One of the most visible signs of aging is the formation of wrinkles and age spots, which are often stimulated by UV radiation and the associated increase in ROS levels. 

Vitamin C plays a key role in maintaining youthful and healthy skin by stimulating collagen synthesis (4). 

Collagen is a vital protein that provides structure, firmness and elasticity to the skin and joints.  As we age, collagen production naturally decreases, leading to sagging skin and wrinkles. Vitamin C functions as a vital cofactor for specific enzymes involved in the adding a hydroxyl group (−OH) (hydroxylation) to  proline and lysine (two important amino acids) residues in collagen molecules.

Why is this important?

The hydroxylation process is essential for several reasons:

Stability of Collagen: The hydroxylation of proline and lysine residues helps stabilize the collagen structure by forming hydrogen bonds between the collagen strands, enhancing the strength and stability of the collagen fibers (6).

Formation of Cross-links: Hydroxylated lysine can participate in cross-linking with other collagen molecules, further enhancing the mechanical properties of the collagen matrix (7).

Triple Helix Formation: Hydroxylation allows procollagen molecules to fold into a precursor of collagen that is then extracted by fibroblasts in the extracellular matrix (8).

In addition to its role in collagen synthesis, Vitamin C scavenges ROS, thereby helping to maintain the integrity of the collagen matrix and preventing degradation by enzymes that break down collagen (9).

In turn, Vitamin E complements this action by increasing skin hydration and protecting the skin from sun damage. It helps maintain the integrity of cell membranes, in part because it prevents lipids from oxidizing, so skin cells retain moisture and stay healthy. This moisturization is very important in keeping the skin firm and youthful (10). 

Changes in skin tone and texture

In addition to preventing wrinkles and age spots, the synergy between Vitamin C and E also contributes to an even skin tone and improved texture. Vitamin C is known for its ability to inhibit melanin production, which can help lighten hyperpigmentation and promote a more uniform skin tone. This is particularly beneficial for those who struggle with dark spots or uneven pigmentation (11).

On the other hand, Vitamin E helps to soothe and calm inflamed skin, reducing redness and irritation. This calming effect not only improves the overall appearance of the skin but also supports its natural barrier function, allowing it to better withstand environmental aggressors (12).

Enhanced Protection Against Environmental Damage

Environmental stressors, including UV radiation and pollutants leads to oxidative damage and accelerate the aging process, and its clear that the combination of vitamins C and E offers a robust defense against this oxidative damage, However, several additional pathways contribute to the synergistic effects of vitamins C and E in enhancing protection against environmental damage:

• Antioxidant Signaling

When UV radiation damages skin cells, it triggers inflammatory pathways that can lead to further skin damage and aging. The antioxidant actions of Vitamins C and E can modulate these inflammatory responses by neutralizing ROS, which are signaling molecules that initiate inflammation. In animal studies, a significant decrease in pro-inflammatory cytokines was observed in groups that received Vitamin C and Vitamin E supplementation (13).

• Gene Expression Modulation

Vitamin C influences the expression of genes involved in the antioxidant defense system, including those that encode superoxide dismutase and glutathione peroxidase. By enhancing the endogenous antioxidant capacity of skin cells, Vitamin C provides further protection against damage caused by UV exposure (14).

• Collagen Synthesis

When exposed to UV radiation, collagen can become damaged. The protective role of Vitamin C not only prevents damage to existing collagen but also supports the production of new collagen, thereby maintaining skin integrity (15).

• Enhanced Cell Survival

Both vitamins contribute to the survival of skin cells under stress. For instance, Vitamin C enhances the resilience of keratinocytes, the primary cell type in the epidermis, against UV-induced apoptosis (programmed cell death). This protection ensures that the skin retains its barrier function and structural integrity even after exposure to harmful UV rays (16).

• Cell Signaling

Vitamins C and E can modulate signaling pathways that respond to oxidative stress. For example, they influence the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) pathway, which is involved in the inflammatory response. By regulating this pathway, these vitamins help prevent chronic inflammation, which can lead to premature skin aging and other skin-related issues (17).

• Epidermal Barrier Maintenance

The skin's barrier function is crucial for protecting against external pollutants. Vitamins C and E contribute to maintaining this barrier by supporting the integrity of the skin's lipid layer.

Beyond Skin Health: Immune and Other Benefits

We observed the ability of Vitamins C and E to influence immune pathways that protect cells from UV rays and environmental stressors associated with aging. But how else does the synergy of these vitamins affect the immune system and other bodily functions?

Immune Function Enhancement

Vitamins C and E are known for their vital role in immune defenses (18): 

• They stimulate the production of white blood cells, particularly lymphocytes and phagocytes, which help the body fight off infections. 
• Additionally, Vitamin C and Vitamin E enhance the skin's barrier function, providing an initial line of defense against pathogens. 

Brain, Heart, and Longevity Benefits

Beyond their roles in skin and immune health, vitamins C and E contribute to various systemic benefits. 

• Vitamin C is crucial for the synthesis of neurotransmitters, which are essential for proper brain function (19). 
• Vitamin C also plays a role in iron absorption, helping to prevent anemia and promote energy levels.
• Vitamin E, being a fat-soluble antioxidant, helps protect lipids (besides membrane ones) in the body from becoming damaged, contributing to cardiovascular health. It also supports healthy blood circulation and may reduce the risk of chronic diseases, such as heart disease and certain types of cancer (20).

The Case for Vitamin C and E Supplements

While a balanced diet rich in fruits and vegetables is essential for overall health, relying solely on dietary intake for vitamins C and E may not always be sufficient to achieve optimal skin health outcomes.

For those looking to boost their intake of these vitamins through food, some of the best sources include fruits, vegetables, nuts, and seeds.

Vitamin C Foods:

• Citrus fruits (like oranges and grapefruits)
• Berries (such as strawberries and blueberries)
• Bell peppers
• Kiwi
• Broccoli

Vitamin E Foods:

• Nuts (especially almonds and hazelnuts)
• Seeds (like sunflower seeds)
• Spinach and other leafy greens
• Avocados
• Fortified cereals

Limitations of Diet Alone

Supplements offer a convenient way to ensure consistent and concentrated doses of these essential vitamins, allowing for targeted benefits that dietary sources may not provide. 

For instance, whole foods such as oranges, strawberries, almonds, and spinach are excellent sources of Vitamin C and E, but the amounts consumed may vary significantly based on personal dietary habits, allergies, seasonality, and growing conditions

Additionally, factors such as food processing and storage can reduce the bioavailability of these nutrients in whole foods. Even so, you need at least 15 bell peppers to achieve a daily intake of Vitamin C, or 10 avocados to meet your daily Vitamin E needs. 

Moreover, during times of increased oxidative stress—such as exposure to UV radiation or pollution—your body's demand for these vitamins may exceed what can be provided through diet alone.  This is where supplementation comes into play.

Jung's Cellular Skin Renewal is a blend of vitamins and advanced, skin-supporting ingredients designed for a complete transformation from the inside out. The formula combines Vitamins C and E, creating a unique synergy that not only protects the skin’s surface but also works beneath it to keep it youthful and firm.

Vitamins C and E are further complemented by a thoughtfully selected array of powerful ingredients:

• Collagen Peptides to improve skin firmness and elasticity
• Astaxanthin for additional antioxidant protection
• Ceramides and Hyaluronic Acid for deep hydration and moisture retention
• N-Acetyl Glucosamine and Niacinamide to even out tone and smooth texture
• Spermidine HCL to support cellular renewal

These ingredients work harmoniously to defend against early signs of aging and environmental damage, helping skin stay youthful and vibrant.

Proactively caring for your skin before the first signs of aging appear is essential, as is understanding which supplements best suit your unique needs. We recommend consulting a healthcare professional before starting any new supplement regimen.

References:

1. Cellular ROS and Antioxidants: Physiological and Pathological Role (2024). Antioxidants.
2. Study of the antioxidant effect of α-tocopherol on low-density lipoprotein peroxidation induced at low and high γ-radiation dose rates (2005). Radiation Physics and Chemistry.
3. The role of vitamin E in normal and damaged skin (1995). Journal of Molecular Medicine. 
4. The Roles of Vitamin C in Skin Health (2017). Nutrients.
5. Antioxidants in Photoaging: From Molecular Insights to Clinical Applications (2024). International Journal of Molecular Science.
6. Proline hydroxylation in collagen supports integrin binding by two distinct mechanisms (2018). Journal of Biological Chemistry.
7. The effect of ascorbic acid on the cross-linking of collagen during its synthesis by cultured 3 T6 fibroblasts (1972). Biochimica et Biophysica Acta.
8. Molecular insights into prolyl and lysyl hydroxylation of fibrillar collagens in health and disease (2017). Critical Reviews in Biochemistry and Molecular Biology.
9. Ascorbic Acid Is a Potential Inhibitor of Collagenases—In Silico and In Vitro Biological Studies (2019). In Silico Drug Design.
10. A Collagen Supplement Improves Skin Hydration, Elasticity, Roughness, and Density: Results of a Randomized, Placebo-Controlled, Blind Study (2019). Nutrients. 
11. Vitamin C prevents ultraviolet-induced pigmentation in healthy volunteers: Bayesian meta-analysis results from 31 randomized controlled versus vehicle clinical studies (2024). Chemical Engineering Research and Design.
12. Vitamin E, oxidative stress, and inflammation (2005). Annual Review of Nutrition.
13. Effects of Vitamin C or E on the Pro-inflammatory Cytokines, Heat Shock Protein 70 and Antioxidant Status in Broiler Chicks under Summer Conditions (2014). Asian-Australasian Journal of Animal Sciences.
14. Alteration in the glutathione, glutathione peroxidase, superoxide dismutase and lipid peroxidation by ascorbic acid in the skin of mice exposed to fractionated γ radiation (2003). Clinica Chimica Acta.
15. The Roles of Vitamin C in Skin Health (2017). Nutrients. 
16. Characterization of keratinocyte differentiation induced by ascorbic acid: protein kinase C involvement and vitamin C homeostasis1 (2002). Journal of Investigative Dermatology.
17. The NF-κB Transcriptional Network Is a High-Dose Vitamin C-Targetable Vulnerability in Breast Cancer (2023). Biomedicines.
18. Antioxidant Vitamins And Their Effect On Immune System (2021). Journal of Physics: Conference Series.
19. Neurobiology of vitamin C: Expanding the focus from antioxidant to endogenous neuromodulator (2019). Pharmacological Research.
20.  The Role of Vitamin E in Human Health and Some Diseases (2014). Sultan Qaboos University Medical Journal.
21. Vitamin E Supplementation Delays Cellular Senescence In Vitro (2015). BioMed Research International.